Device

Part:BBa_K299817

Designed by: Michał Lower   Group: iGEM10_Warsaw   (2010-10-13)

This part is licensed under
Creative BioCommons

control construct - GFP under J23102


Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from Aequorea victoria.

Authors:

Cloned by Joanna Leszczyńska.

Work supervised by Michał Lower.

Construct design

The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 708


[edit]
Categories
Parameters
n/acontrol construct - GFP under J23102